| Fluorescence microscopy image of the same cells. The blue fluorescent label localizes to DNA, highlighting cell nuclei. The green fluorescent label localizes to a protein found only in dendrites, a neural substructure. The red fluorescent label localizes to a protein found only in axons, another neural substructure. With these labels it is much easier to understand what’s happening in the sample. For example, the green and red labels in Outset 1 confirm this is a neural cluster. The red label in Outset 3 shows that the neurites are axons, not dendrites. The upper-left blue dot in Outset 4 reveals a previously hard-to-see nucleus, and the lack of a blue dot for the cell at the left shows it to be DNA-free cellular debris. |
